SubtiBank SubtiBank
ytiB [2019-08-09 07:49:03]
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ytiB [2019-08-09 07:49:03]

similar to carbonic anhydrase
locus
BSU_30690
pI
6.17
mw
20.97 kDa
protein length
187 aa Sequence Blast
gene length
564 bp Sequence Blast
function
unknown
product
unknown
essential
no
ec
4.2.1.1
synonyms

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    3,138,321 → 3,138,884

    The protein

    Catalyzed reaction/ biological activity

  • H2CO3 = CO2 H2O (according to Swiss-Prot)
  • Protein family

  • beta-class carbonic anhydrase family (with [protein|E00889289B07BE78CC0C8E0962A55C3B14871E00|YvdA] and [protein|5D96DF2F32050BBCEC3811843398FE84DE88F6DF|YbcF], according to UniProt)
  • Paralogous protein(s)

  • [protein|E00889289B07BE78CC0C8E0962A55C3B14871E00|YvdA]
  • [protein|5D96DF2F32050BBCEC3811843398FE84DE88F6DF|YbcF]: (32%)
  • Structure

  • [PDB|1G5C] (from Methanobacterium thermoautotrophicum, 34% identity) [pubmed|11096105]
  • Expression and Regulation

    Operons

    genes
    [gene|F942C0F4618FACEF35ACA629355BAC288556E7B6|ytiB]-[gene|FF61DC6CA162EEC191CDFC802E721FCBD32EC098|luxS]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|26577401], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
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    Biological materials

    Mutant

  • MGNA-A293 (ytiB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/293 NBRP B. subtilis, Japan]
  • BKE30690 (Δ[gene|F942C0F4618FACEF35ACA629355BAC288556E7B6|ytiB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCGTTGTCCCTTTCT, downstream forward: _UP4_CTTATCGTAAACGGTTATAA
  • BKK30690 (Δ[gene|F942C0F4618FACEF35ACA629355BAC288556E7B6|ytiB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCGTTGTCCCTTTCT, downstream forward: _UP4_CTTATCGTAAACGGTTATAA
  • References

  • 26577401,11096105